N6AMT1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
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Primary Accession | Q9Y5N5 |
Other Accession | NP_037372.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 22957 Da |
Antigen Region | 1-30 aa |
Gene ID | 29104 |
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Other Names | HemK methyltransferase family member 2, 211-, MHsaHemK2P, N(6)-adenine-specific DNA methyltransferase 1, N6AMT1, C21orf127, HEMK2 |
Target/Specificity | This N6AMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human N6AMT1. |
Dilution | WB~~1:2000 FC~~1:25 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | N6AMT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | N6AMT1 {ECO:0000303|PubMed:30017583, ECO:0000312|HGNC:HGNC:16021} |
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Function | Methyltransferase that can methylate proteins and, to a lower extent, arsenic (PubMed:18539146, PubMed:21193388, PubMed:30017583, PubMed:31636962, PubMed:31061526). Catalytic subunit of a heterodimer with TRMT112, which monomethylates 'Lys-12' of histone H4 (H4K12me1), a modification present at the promoters of numerous genes encoding cell cycle regulators (PubMed:31061526). Catalytic subunit of a heterodimer with TRMT112, which catalyzes N5-methylation of Glu residue of proteins with a Gly-Gln-Xaa-Xaa-Xaa-Arg motif (PubMed:18539146, PubMed:31632689, PubMed:31636962). Methylates ETF1 on 'Gln-185'; ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated (PubMed:18539146, PubMed:20606008, PubMed:31636962, PubMed:31061526). May also play a role in the modulation of arsenic-induced toxicity by mediating the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid (PubMed:21193388, PubMed:25997655). It however only plays a limited role in arsenic metabolism compared with AS3MT (PubMed:25997655). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q6SKR2}. |
Tissue Location | Widely expressed, with highest expression in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest expression in leukocytes and mammary gland |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene belongs to the methyltransferase superfamily. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Figaro, S., et al. FEBS Lett. 582(16):2352-2356(2008)
Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007) :
Hwang, S.J., et al. BMC Med. Genet. 8 SUPPL 1, S10 (2007) :
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