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GTF2I Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • IF - GTF2I Antibody (N-term) AP18119a
    Fluorescent image of Hela cell stained with GTF2I Antibody (N-term)(Cat#AP18119a/SA111012BI).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). GTF2I immunoreactivity is localized to Nucleus significantly.
  • WB - GTF2I Antibody (N-term) AP18119a
    GTF2I Antibody (N-term) (Cat. #AP18119a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P78347
Other Accession Q5U2Y1, Q9ESZ8, NP_001157108.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 112416 Da
Antigen Region 281-310 aa
Additional Information
Gene ID 2969
Other Names General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, BAP-135, BTK-associated protein 135, SRF-Phox1-interacting protein, SPIN, Williams-Beuren syndrome chromosomal region 6 protein, GTF2I, BAP135, WBSCR6
Target/Specificity This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the N-terminal region of human GTF2I.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGTF2I Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GTF2I
Synonyms BAP135, WBSCR6
Function Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00484, ECO:0000269|PubMed:10373551}. Note=Colocalizes with BTK in the cytoplasm
Tissue Location Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues
Research Areas
Citations (0)

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This gene encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 7, 13 and 21.


Antonell, A., et al. J. Med. Genet. 47(5):312-320(2010)
Lazebnik, M.B., et al. J. Biol. Chem. 284(52):36234-36239(2009)
Sacristan, C., et al. Eur. J. Immunol. 39(9):2584-2595(2009)
Makeyev, A.V., et al. Gene 433 (1-2), 16-25 (2009) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 295.00
$ 99.00
Cat# AP18119a
(40 western blots)
Availability: In Stock
Bulk Size
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