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SNAP25 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SNAP25 Antibody (Center) AP18126C
    Anti-SNAP25 Antibody (Center) at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60880
Other Accession P60881, P60879, P60878, Q17QQ3, Q6PC54, Q5TZ66, NP_003072.2
Reactivity Human
Predicted Zebrafish, Bovine, Chicken, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23315 Da
Antigen Region 36-63 aa
Additional Information
Gene ID 6616
Other Names Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP25, SNAP
Target/Specificity This SNAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the Central region of human SNAP25.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSNAP25 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNAP25
Synonyms SNAP
Function t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Cellular Location Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome Note=Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region (By similarity).
Tissue Location Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum
Research Areas
Citations (0)

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Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.


Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Greaves, J., et al. J. Biol. Chem. 285(32):24629-24638(2010)
Oner, O., et al. J Atten Disord (2010) In press :
Zhang, H., et al. Eur. J. Paediatr. Neurol. (2010) In press :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

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$ 295.00
$ 99.00
Cat# AP18126C
(40 western blots)
Availability: In Stock
Bulk Size
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