|Application ||WB, E|
|Other Accession||Q9Z0N2, P81795, Q9Z0N1, Q5ZMS3, Q2KHU8, Q2VIR3, NP_001406.1, F1QGW6, C9WPN6|
|Predicted||Bovine, Chicken, Zebrafish, Mouse, Rat|
|Calculated MW||51109 Da|
|Antigen Region||364-391 aa|
|Other Names||Eukaryotic translation initiation factor 2 subunit 3, Eukaryotic translation initiation factor 2 subunit gamma X, eIF-2-gamma X, eIF-2gX, EIF2S3, EIF2G|
|Target/Specificity||This EIF2S3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-391 amino acids from the C-terminal region of human EIF2S3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF2S3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (By similarity).|
|Tissue Location||Expressed in testis, brain, liver and muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit. [provided by RefSeq].
Burkhardt, J., et al. J. Rheumatol. 36(10):2149-2157(2009)
Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Mikami, S., et al. Protein Expr. Purif. 46(2):348-357(2006)
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