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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   KLH22 Antibody (C-term)   

KLH22 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - KLH22 Antibody  (C-term) AP18159b
    KLH22 Antibody (C-term) (Cat. #AP18159b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KLH22 antibody detected the KLH22 protein (arrow).
  • WB - KLH22 Antibody  (C-term) AP18159b
    KLH22 Antibody (C-term) (Cat. #AP18159b) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the KLH22 antibody detected the KLH22 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q53GT1
Other Accession NP_116164.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 71667 Da
Antigen Region 586-614 aa
Additional Information
Gene ID 84861
Other Names Kelch-like protein 22, KLHL22
Target/Specificity This KLH22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-614 amino acids from the C-terminal region of human KLH22.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLH22 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLHL22
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase
Research Areas
Citations (0)

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Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for cell division. BCR E3 ubiquitin ligase complexes mediate the ubiquitination of target proteins.


Maerki, S., et al. J. Cell Biol. 187(6):791-800(2009)
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :

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$ 295.00
$ 99.00
Cat# AP18159b
(40 western blots)
Availability: In Stock
Bulk Size
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