|Application ||WB, E|
|Calculated MW||71667 Da|
|Antigen Region||586-614 aa|
|Other Names||Kelch-like protein 22, KLHL22|
|Target/Specificity||This KLH22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-614 amino acids from the C-terminal region of human KLH22.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KLH22 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase|
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Provided below are standard protocols that you may find useful for product applications.
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for cell division. BCR E3 ubiquitin ligase complexes mediate the ubiquitination of target proteins.
Maerki, S., et al. J. Cell Biol. 187(6):791-800(2009)
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
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