- CITATIONS: 1
|Application ||IHC-P, E|
|Calculated MW||25279 Da|
|Antigen Region||15-45 aa|
|Other Names||Ubiquitin-like-conjugating enzyme ATG10, 632-, Autophagy-related protein 10, APG10-like, ATG10, APG10L|
|Target/Specificity||This ATG10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~45 amino acids from the N-term of human APG10L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATG10 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.|
Provided below are standard protocols that you may find useful for product applications.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG10 is an ATG12-conjugating enzyme (E2-like enzyme) that likely serves as an ATG5-recognition molecule. This protein interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. APG10 also is able to directly interact either with ATG5 or ATG7.
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Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
Greenberg JT. Dev Cell. 8(6):799-801. (2005)
Levine B. Cell. 120(2):159-62. (2005)
Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
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