- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
ATG12 AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP1816c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ATG12 Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | O94817 |
| Other Accession | Q2TBJ5, Q9CQY1, Q3T0W7 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 15113 Da |
ATG12 Antibody - Additional info | |
| Gene ID 9140 | |
| Other Names ATG12; APG12; APG12L; Ubiquitin-like protein ATG12; Autophagy-related protein 12 | |
| Target/Specificity This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105~135 amino acids surrounding P46 of human APG12L. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG12 Antibody - Protein Information | |
| Name ATG12 | |
| Synonyms APG12, APG12L | |
| Function Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5 | |
| Cellular Location Cytoplasm (By similarity). | |
| Tissue Location Ubiquitous. | |
ATG12 Antibody - Related products
AP1151a: ATG12 Antibody (N-term)
AP1816a: ATG12 Antibody (N-term)
RI10451: ATG12 predesign siRNA
DC03063: Human ATG12 cDNA Clone
BP1151a: ATG12 Antibody (N-term) Blocking Peptide
BP1151b: Phospho-Histone H3-S10 Antibody Blocking Peptide
BP1151c: Phospho-Histone H3-S10 Antibody Blocking Peptide
ATG12 Antibody - Application data
-
Western blot analysis of hAPG12L-P66 (Cat. #AP1816c) in mouse brain tissue lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.
-
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
-
APG12L Antibody (Cat. #AP1816c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the APG12L antibody detected the APG12L protein (arrow).
ATG12 Antibody - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
REFERENCES
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
