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ATG16L AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1817d | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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- Backgrounds
ATG16L Antibody - Product info | |
| Application | IF, WB
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| Primary Accession | Q676U5 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 68265 Da |
ATG16L Antibody - Additional info | |
| Gene ID 55054 | |
| Other Names ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1 | |
| Target/Specificity This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human APG16. | |
| Dilution IF~~1:100 WB~~1:50~100WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG16L Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG16L Antibody - Protein Information | |
| Name ATG16L1 | |
| Synonyms APG16L | |
| Function Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II | |
| Cellular Location Cytoplasm (By similarity). Preautophagosomal structure membrane; Peripheral membrane protein (By similarity) Note=Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity) | |
ATG16L Antibody - Related products
AM1817a: ATG16L1 Antibody (Ascites)
AP1817a: ATG16L Antibody (N-term)
AP1817c: ATG16L Antibody (C-term)
AP3366a: Phospho-ATG16L-S287 Antibody
AP3442a: Phospho-ATG16L-S213 Antibody
RI10452: ATG16L1 predesign siRNA
BP1817b: APG16L Antibody (L176) Blocking Peptide
BP1817c: APG16L Antibody (C-term) Blocking Peptide
BP1817d: APG16 Antibody Blocking Peptide
ATG16L Antibody - Application data
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Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1817d ATG16L primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978).
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Western blot analysis of anti-APG16 Pab (Cat.#AP1817d) in Hela cell line lysates (35ug/lane).APG16(arrow) was detected using the purified Pab (1:60 dilution).
ATG16L Antibody - Research Areas
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BACKGROUND
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.
REFERENCES
References for protein: 1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) 2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005) 4.Levine B. Cell. 120(2):159-62. (2005) 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) References for U251 cell line: 1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
