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ENTPD4 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9Y227 |
| Other Accession | NP_001122402.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 70255 Da |
| Antigen Region | 40-69 aa |
| Gene ID | 9583 |
|---|---|
| Other Names | Ectonucleoside triphosphate diphosphohydrolase 4, NTPDase 4, Lysosomal apyrase-like protein of 70 kDa, Uridine-diphosphatase, UDPase, ENTPD4, KIAA0392, LALP70, LYSAL1 |
| Target/Specificity | This ENTPD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human ENTPD4. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ENTPD4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ENTPD4 (HGNC:14573) |
|---|---|
| Function | [Isoform 1]: Catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with a preference for pyrimidines. Preferentially hydrolyzes UTP and TTP. AMP, ADP, ATP and UMP are not substrates (PubMed:10858452, PubMed:9556635). Preferentially activated by Ca(2+) over Mg(2+) (PubMed:10858452). |
| Cellular Location | [Isoform 1]: Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi- pass membrane protein |
| Tissue Location | Ubiquitous. Highest expression in testis and lowest in bladder. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
ENTPD4 hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent. The order of activity with different substrates is UDP >> GDP = CDP = TDP, AMP, ADP, ATP and UMP are not substrates. Preferred substrates for isoform 2 are CTP, UDP, CDP, GTP and GDP, while isoform 1 utilizes UTP and TTP.
References
Saito, A., et al. Psychiatry Res (2010) In press :
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Biederbick, A., et al. BMC Biochem. 5, 8 (2004) :
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