|Application ||WB, E|
|Calculated MW||38930 Da|
|Antigen Region||77-105 aa|
|Other Names||Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo, IZUMO1|
|Target/Specificity||This IZUM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human IZUM1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IZUM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction|
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Provided below are standard protocols that you may find useful for product applications.
The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).
Ellerman, D.A., et al. Mol. Reprod. Dev. 76(12):1188-1199(2009)
Inoue, N., et al. Biochem. Biophys. Res. Commun. 377(3):910-914(2008)
Granados-Gonzalez, V., et al. Fertil. Steril. 90(4):1246-1248(2008)
Yatsenko, A.N., et al. Hum. Mol. Genet. 15(23):3411-3419(2006)
Inoue, N., et al. Nature 434(7030):234-238(2005)
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