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IZUM1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8IYV9 |
| Other Accession | NP_872381.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 38930 Da |
| Antigen Region | 77-105 aa |
| Gene ID | 284359 |
|---|---|
| Other Names | Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo, IZUMO1 |
| Target/Specificity | This IZUM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human IZUM1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | IZUM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | IZUMO1 {ECO:0000303|PubMed:15759005, ECO:0000312|HGNC:HGNC:28539} |
|---|---|
| Function | Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human- specific oolemma epitope FCRL3/MAIA during fertilization (PubMed:36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed:36070373). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein. Note=Localizes initially to the acrosome membrane of the sperm head (both outer and inner acrosomal membranes) (PubMed:36070373). During the acrosome reaction, translocates to the plasma membrane (PubMed:15759005, PubMed:36070373). |
| Tissue Location | Sperm-specific (at protein level) (PubMed:15759005, PubMed:36070373). Detectable on sperm surface only after the acrosome reaction (PubMed:15759005, PubMed:36070373) |
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Provided below are standard protocols that you may find useful for product applications.
Background
The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).
References
Ellerman, D.A., et al. Mol. Reprod. Dev. 76(12):1188-1199(2009)
Inoue, N., et al. Biochem. Biophys. Res. Commun. 377(3):910-914(2008)
Granados-Gonzalez, V., et al. Fertil. Steril. 90(4):1246-1248(2008)
Yatsenko, A.N., et al. Hum. Mol. Genet. 15(23):3411-3419(2006)
Inoue, N., et al. Nature 434(7030):234-238(2005)
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