|Application ||WB, E|
|Calculated MW||38930 Da|
|Antigen Region||77-105 aa|
|Other Names||Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo, IZUMO1|
|Target/Specificity||This IZUM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human IZUM1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IZUM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Sperm-specific (at protein level). Detectable on sperm surface only after the acrosome reaction|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).
Ellerman, D.A., et al. Mol. Reprod. Dev. 76(12):1188-1199(2009)
Inoue, N., et al. Biochem. Biophys. Res. Commun. 377(3):910-914(2008)
Granados-Gonzalez, V., et al. Fertil. Steril. 90(4):1246-1248(2008)
Yatsenko, A.N., et al. Hum. Mol. Genet. 15(23):3411-3419(2006)
Inoue, N., et al. Nature 434(7030):234-238(2005)
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