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Beclin 1 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Beclin 1 Antibody AP1818a
    Western blot analysis of anti-hBECN1-Q196 Pab (Cat. #AP1818a) in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-Q196(arrow) was detected using the purified Pab.
  • WB - Beclin 1 Antibody AP1818a
    The anti-BECN1 Pab (Cat. #AP1818a) is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified Pab.
  • IHC-P - Beclin 1 Antibody AP1818a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14457
Other Accession Q5ZKS6, Q4A1L4
Reactivity Human, Mouse
Predicted Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51896 Da
Antigen Region 181-210 aa
Additional Information
Gene ID 8678
Other Names Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Target/Specificity This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from human Beclin 1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeclin 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BECN1
Synonyms GT197
Function Plays a central role in autophagy (PubMed:23184933). Required for the abcission step in cytokinesis (PubMed:20208530). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O88597}. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19050071}
Tissue Location Ubiquitous.
Research Areas
Citations ( 0 )


Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.


Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) Liang,X.H., et al. J. Virol. 72 (11), 8586-8596 (1998) Aita V.M., et al. Genomics 59:59-65(1999).

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$ 295.00
$ 99.00
Cat# AP1818a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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