|Application ||WB, E|
|Other Accession||A0JPP1, Q9D6N5, Q2YDP3, NP_006433.2|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||22350 Da|
|Antigen Region||1-29 aa|
|Other Names||Dr1-associated corepressor, Dr1-associated protein 1, Negative cofactor 2-alpha, NC2-alpha, DRAP1|
|Target/Specificity||This DRAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human DRAP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DRAP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.|
|Tissue Location||Ubiquitous. Highly expressed in adult testis, heart, skeletal muscle, pancreas and brain, and in fetal brain, liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex.
Kahle, J., et al. J. Biol. Chem. 284(14):9382-9393(2009)
Schluesche, P., et al. Nat. Struct. Mol. Biol. 14(12):1196-1201(2007)
Albert, T.K., et al. Proc. Natl. Acad. Sci. U.S.A. 104(24):10000-10005(2007)
Lim, J., et al. Cell 125(4):801-814(2006)
Assmann, E.M., et al. J. Biol. Chem. 281(15):9869-9881(2006)
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