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GABARAPL2 Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GABARAPL2  Antibody AP1822d
    Western blot analysis of GABARAPL2 Antibody (Cat. #AP1822d) in MCF-7 cell line lysates (35ug/lane). GABARAPL2 (arrow) was detected using the purified Pab.
  • IHC-P - GABARAPL2  Antibody AP1822d
    Formalin-fixed and paraffin-embedded mouse brain tissue reacted with GABARAPL2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - GABARAPL2  Antibody AP1822d
    GABARAPL2 Antibody (Cat.#AP1822d) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60520
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13667 Da
Additional Information
Gene ID 11345
Other Names Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, GATE-16, MAP1 light chain 3-related protein, GABARAPL2, FLC3A, GEF2
Target/Specificity This GABARAPL2 antibody is generated from rabbits immunized with human GABARAPL2 recombinant protein.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGABARAPL2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms FLC3A, GEF2
Function Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Cellular Location Golgi apparatus. Cytoplasmic vesicle, autophagosome
Tissue Location Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle Expressed at very low levels in lung, thymus and small intestine
Research Areas
Citations ( 0 )


Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.


Sou,Y.S., J. Biol. Chem. 281 (6), 3017-3024 (2006)
Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)
Wiemann,S., Genome Res. 14 (10B), 2136-2144 (2004)
Sagiv,Y., EMBO J. 19 (7), 1494-1504 (2000)

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$ 295.00
$ 99.00
Cat# AP1822d
(40 western blots)
Availability: In Stock
Bulk Size
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