|Application ||WB, E|
|Calculated MW||151557 Da|
|Antigen Region||1152-1178 aa|
|Other Names||Leucine-rich repeat-containing protein 16A, CARMIL homolog, LRRC16A, CARMIL, CARMIL1a, LRRC16|
|Target/Specificity||This LRRC16A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1178 amino acids from the C-terminal region of human LRRC16A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LRRC16A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CARMIL, CARMIL1a, LRRC16|
|Function||Binds CAPZA2 with high affinity and significantly decreases CAPZA2 affinity for actin barbed ends. Increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Rapidly uncaps barbed ends capped by CAPZA2 and enhances barbed-end actin polymerization (By similarity). May control actin dynamics in lamellipodia. Required for cell migration.|
|Cellular Location||Cell projection, lamellipodium. Cytoplasm. Note=Colocalized with F-actin in lamellipodia but not with F-actin in stress fibers. Also found on macropinosomes|
|Tissue Location||Expressed in lung, placenta, small intestine, liver, thymus, colon, skeletal muscle, heart and brain. Higher expression in kidney.|
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Provided below are standard protocols that you may find useful for product applications.
LRRC16A binds CAPZA2 with high affinity and significantly decreases CAPZA2 affinity for actin barbed ends. Increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Rapidly uncaps barbed ends capped by CAPZA2 and enhances barbed-end actin polymerization (By similarity).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Polasek, O., et al. Croat. Med. J. 51(1):32-39(2010)
Gunjaca, G., et al. Croat. Med. J. 51(1):23-31(2010)
van der Harst, P., et al. Hum. Mol. Genet. 19(2):387-395(2010)
Liang, Y., et al. Mol. Biol. Cell 20(24):5290-5305(2009)
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