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LRRC16A Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q5VZK9 |
| Other Accession | NP_060110.4 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 151557 Da |
| Antigen Region | 1152-1178 aa |
| Gene ID | 55604 |
|---|---|
| Other Names | Leucine-rich repeat-containing protein 16A, CARMIL homolog, LRRC16A, CARMIL, CARMIL1a, LRRC16 |
| Target/Specificity | This LRRC16A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1178 amino acids from the C-terminal region of human LRRC16A. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LRRC16A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CARMIL1 {ECO:0000303|PubMed:16054028, ECO:0000312|HGNC:HGNC:21581} |
|---|---|
| Function | Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization, however, seems unable to nucleate filaments (PubMed:16054028). Plays a role in lamellipodial protrusion formations and cell migration (PubMed:19846667). |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q6EDY6}. Cell membrane. Cell projection, lamellipodium. Note=Found on macropinosomes (PubMed:19846667). Colocalized with heterodimeric capping protein (CP) and F-actin in lamellipodia but not with F-actin in stress fibers (PubMed:19846667). |
| Tissue Location | Expressed in lung, placenta, small intestine, liver, thymus, colon, skeletal muscle, heart and brain. Higher expression in kidney. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
LRRC16A binds CAPZA2 with high affinity and significantly decreases CAPZA2 affinity for actin barbed ends. Increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Rapidly uncaps barbed ends capped by CAPZA2 and enhances barbed-end actin polymerization (By similarity).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Polasek, O., et al. Croat. Med. J. 51(1):32-39(2010)
Gunjaca, G., et al. Croat. Med. J. 51(1):23-31(2010)
van der Harst, P., et al. Hum. Mol. Genet. 19(2):387-395(2010)
Liang, Y., et al. Mol. Biol. Cell 20(24):5290-5305(2009)
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