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HAUS8 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HAUS8 Antibody (N-term) AP18272a
    HAUS8 Antibody (N-term) (Cat. #AP18272a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HAUS8 antibody detected the HAUS8 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BT25
Other Accession NP_219485.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44857 Da
Antigen Region 28-54 aa
Additional Information
Gene ID 93323
Other Names HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48, HAUS8, HICE1
Target/Specificity This HAUS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-54 amino acids from the N-terminal region of human HAUS8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHAUS8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HAUS8
Synonyms HICE1
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively. Localizes to mitotic spindle microtubules
Research Areas
Citations (0)
citation

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Background

HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).

References

Wu, G., et al. Mol. Biol. Cell 20(22):4686-4695(2009)
Lawo, S., et al. Curr. Biol. 19(10):816-826(2009)
Uehara, R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(17):6998-7003(2009)
Wu, G., et al. Mol. Cell. Biol. 28(11):3652-3662(2008)
Goshima, G., et al. J. Cell Biol. 181(3):421-429(2008)

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$ 182.50
$ 70.00
Cat# AP18272a
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