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TFE3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - TFE3 Antibody (N-term) AP18316A
    Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TFE3 Antibody (N-term) AP18316A
    TFE3 Antibody (N-term) (Cat. #AP18316a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).
  • WB - TFE3 Antibody (N-term) AP18316A
    TFE3 Antibody (N-term) (Cat. #AP18316a) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).
  • IF - TFE3 Antibody (N-term) AP18316A
    Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term)(Cat#AP18316a). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19532
Other Accession NP_006512
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61521 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 7030
Other Names Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33, TFE3, BHLHE33
Target/Specificity This TFE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TFE3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFE3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFE3
Synonyms BHLHE33
Function Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
Cellular Location Nucleus.
Tissue Location Ubiquitous in fetal and adult tissues.
Research Areas
Citations (0)

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The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3, SMAD3, and LEF-1, and is involved in TGF-beta-induced transcription, playing important roles in cell growth, proliferation, and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene, and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1); it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations, resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO (p54nrb)-TFE3 and ASPL-TFE3.


Argani, P., et al. Am. J. Surg. Pathol. 34(10):1395-1406(2010)
Haudebourg, J., et al. Cancer Genet. Cytogenet. 200(2):75-78(2010)
Chang, I.W., et al. Am. J. Surg. Pathol. 33(12):1894-1901(2009)
Yamaguchi, T., et al. Acta Cytol. 53(6):693-697(2009)
Kuroda, N., et al. Pathol. Int. 59(10):769-770(2009)

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$ 295.00
$ 99.00
Cat# AP18316A
(40 western blots)
Availability: In Stock
Bulk Size
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