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BARON Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BARON Antibody (Center) AP1831c
    All lanes : Anti-BARON Antibody (Center) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - BARON Antibody (Center) AP1831c
    Formalin-fixed and paraffin-embedded human kidney reacted with BARON Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - BARON Antibody (Center) AP1831c
    BARON Antibody (Center) (Cat. #AP1831c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92622
Other Accession Q80U62
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 108622 Da
Antigen Region 656-684 aa
Additional Information
Gene ID 9711
Other Names Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein, Baron, KIAA0226
Target/Specificity This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the Central region of human BARON.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBARON Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KIAA0226
Function Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity (By similarity).
Cellular Location Late endosome. Lysosome. Early endosome. Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus
Research Areas
Citations (0)

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Matsunaga, K., et al. Nat. Cell Biol. 11(4):385-396(2009)
Muzny, D.M., et al. Nature 440(7088):1194-1198(2006)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)

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$ 295.00
$ 99.00
Cat# AP1831c
(40 western blots)
Availability: In Stock
Bulk Size
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