|Application ||WB, IHC-P, FC, E|
|Calculated MW||108622 Da|
|Antigen Region||656-684 aa|
|Other Names||Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein, Baron, KIAA0226|
|Target/Specificity||This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the Central region of human BARON.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BARON Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745).|
|Cellular Location||Late endosome. Lysosome. Early endosome Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus|
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Provided below are standard protocols that you may find useful for product applications.
Matsunaga, K., et al. Nat. Cell Biol. 11(4):385-396(2009)
Muzny, D.M., et al. Nature 440(7088):1194-1198(2006)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)
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