|Application ||WB, E|
|Calculated MW||87640 Da|
|Antigen Region||496-524 aa|
|Other Names||Cyclin-F, F-box only protein 1, CCNF, FBX1, FBXO1|
|Target/Specificity||This CCNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-524 amino acids from the Central region of human CCNF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CCNF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110 Also localizes to the nucleus|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified.
D'Angiolella, V., et al. Nature 466(7302):138-142(2010)
Fung, T.K., et al. J. Biol. Chem. 277(38):35140-35149(2002)
Kong, M., et al. EMBO J. 19(6):1378-1388(2000)
Bai, C., et al. Cell 86(2):263-274(1996)
Bai, C., et al. EMBO J. 13(24):6087-6098(1994)
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