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ANO1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q5XXA6 |
| Other Accession | NP_060513.5 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 114078 Da |
| Antigen Region | 453-482 aa |
| Gene ID | 55107 |
|---|---|
| Other Names | Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2, ANO1, DOG1, ORAOV2, TAOS2, TMEM16A |
| Target/Specificity | This ANO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the Central region of human ANO1. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ANO1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ANO1 |
|---|---|
| Function | Calcium-activated chloride channel (CaCC) (PubMed:20056604, PubMed:22178883, PubMed:22946059, PubMed:32487539). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression (PubMed:28963502). Required for basal and ATP-dependent mucus secretion in airways and intestine, probably by controlling exocytosis of mucus-filled granules by providing Ca(2+) to an apical signaling compartment (By similarity). Contributes to airway mucus expression induced by interleukins IL3 and IL8 and by the asthma-associated protein CLCA1 and is required for expression of mucin MUC5AC (PubMed:33026825). However, was shown in another study not to be required for MUC5AC expression (PubMed:31732694). Plays a role in the propagation of Ca(2+) waves in Kolliker's organ in the cochlea and contributes to the refinement of auditory brainstem circuitries prior to hearing onset (By similarity). In vomeronasal sensory neurons, modulates spontaneous firing patterns in the absence of stimuli as well as the firing pattern of pheromone- evoked activity (By similarity). Responsible for calcium-activated chloride channel activity in type I taste cells of the vallate papillae (By similarity). Acts as a heat sensor in nociceptive neurons (By similarity). In dorsal root ganglion neurons, plays a role in mediating non-histaminergic Mas-related G-protein coupled receptor (MRGPR)- dependent itching, acting as a downstream effector of MRGPRs (By similarity). In the developing brain, required for the Ca(2+)-dependent process extension of radial glial cells (By similarity). |
| Cellular Location | Apical cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q8BHY3}. Presynapse {ECO:0000250|UniProtKB:Q8BHY3}. Note=In differentiating airway epithelial cells, predominantly intracellular at day 0 but is apically localized by day 30. Expressed in the presynapse of retinal neurons (By similarity). {ECO:0000250|UniProtKB:Q8BHY3} |
| Tissue Location | Expressed in nasal epithelial cells (at protein level) (PubMed:32487539). In the kidney, expressed in the collecting duct (at protein level) (PubMed:24913262). Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles (PubMed:16906560, PubMed:15215166). Expressed in eccrine sweat glands (PubMed:25220078). |
Citations (0)
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
ANO1 acts as a calcium-activated chloride channel. Required for normal tracheal development (By similarity).
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Barro-Soria, R., et al. Pflugers Arch. 459(3):485-497(2010)
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Ferrera, L., et al. J. Biol. Chem. 284(48):33360-33368(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
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$ 182.50
$ 70.00
Cat# AP18347c
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