|Application ||WB, E|
|Other Accession||D3ZKD3, Q3TSG4, E1BH29, NP_060228.3|
|Predicted||Mouse, Rat, Bovine|
|Calculated MW||44256 Da|
|Antigen Region||302-330 aa|
|Other Names||RNA demethylase ALKBH5, 11411-, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH5, ABH5, OFOXD1|
|Target/Specificity||This ALKBH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the Central region of human ALKBH5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ALKBH5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)- methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity).|
|Cellular Location||Nucleus speckle|
|Tissue Location||Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary|
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Provided below are standard protocols that you may find useful for product applications.
Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Bi, W., et al. Genome Res. 12(5):713-728(2002)
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