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ALKBH5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ALKBH5 Antibody (Center) AP18410c
    Anti-ALKBH5 Antibody (Center) at 1:2000 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ALKBH5 Antibody (Center) AP18410c
    All lanes : Anti-ALKBH5 Antibody (Center) at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6P6C2
Other Accession D3ZKD3, Q3TSG4, E1BH29, NP_060228.3
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44256 Da
Antigen Region 302-330 aa
Additional Information
Gene ID 54890
Other Names RNA demethylase ALKBH5, 11411-, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH5, ABH5, OFOXD1
Target/Specificity This ALKBH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the Central region of human ALKBH5.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALKBH5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ABH5, OFOXD1
Function Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)- methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity).
Cellular Location Nucleus speckle
Tissue Location Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary
Citations (0)

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Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).


Olsen, J.V., et al. Cell 127(3):635-648(2006)
Bi, W., et al. Genome Res. 12(5):713-728(2002)

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Cat# AP18410c
(40 western blots)
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