|Application ||WB, E|
|Calculated MW||63267 Da|
|Antigen Region||1-30 aa|
|Other Names||Non-canonical poly(A) RNA polymerase PAPD5, PAP-associated domain-containing protein 5, Terminal uridylyltransferase 3, TUTase 3, Topoisomerase-related function protein 4-2, TRF4-2, PAPD5|
|Target/Specificity||This PAPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PAPD5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PAPD5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post- transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Predominantly expressed in the cytoplasm (PubMed:18172165)|
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Provided below are standard protocols that you may find useful for product applications.
PAPD5 plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Mullen, T.E., et al. Genes Dev. 22(1):50-65(2008)
Walowsky, C., et al. J. Biol. Chem. 274(11):7302-7308(1999)
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