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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PPP4R2 Antibody (Center)   

PPP4R2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPP4R2 Antibody (Center) AP18485c
    PPP4R2 Antibody (Center) (Cat. #AP18485c) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the PPP4R2 antibody detected the PPP4R2 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NY27
Other Accession NP_777567.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46898 Da
Antigen Region 228-254 aa
Additional Information
Gene ID 151987
Other Names Serine/threonine-protein phosphatase 4 regulatory subunit 2, PPP4R2
Target/Specificity This PPP4R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the Central region of human PPP4R2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP4R2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP4R2
Function Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Note=Ionizing radiation induces relocalization to nuclear foci and colocalization with RPA2
Tissue Location Widely expressed.
Research Areas
Citations (0)

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Background

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.

References

Lee, D.H., et al. Nat. Struct. Mol. Biol. 17(3):365-372(2010)
Chen, G.I., et al. J. Biol. Chem. 283(43):29273-29284(2008)
Chowdhury, D., et al. Mol. Cell 31(1):33-46(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Gingras, A.C., et al. Mol. Cell Proteomics 4(11):1725-1740(2005)

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$ 295.00
$ 99.00
Cat# AP18485c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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