|Application ||WB, E|
|Calculated MW||46898 Da|
|Antigen Region||228-254 aa|
|Other Names||Serine/threonine-protein phosphatase 4 regulatory subunit 2, PPP4R2|
|Target/Specificity||This PPP4R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the Central region of human PPP4R2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PPP4R2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Note=Ionizing radiation induces relocalization to nuclear foci and colocalization with RPA2|
|Tissue Location||Widely expressed.|
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Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
Lee, D.H., et al. Nat. Struct. Mol. Biol. 17(3):365-372(2010)
Chen, G.I., et al. J. Biol. Chem. 283(43):29273-29284(2008)
Chowdhury, D., et al. Mol. Cell 31(1):33-46(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Gingras, A.C., et al. Mol. Cell Proteomics 4(11):1725-1740(2005)
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