- CITATIONS: 7
|Application ||WB, IHC-P, IF, E|
|Calculated MW||78151 Da|
|Antigen Region||666-699 aa|
|Other Names||UV radiation resistance-associated gene protein, p63, UVRAG|
|Target/Specificity||This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-699 amino acids from the C-terminal region of human UVRAG.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UVRAG Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Versatile protein that is involved in regulation of differenent cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed:24056303). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2 (PubMed:16799551, PubMed:20643123, PubMed:24056303). Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events (PubMed:18552835). Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion (PubMed:24550300). In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly (PubMed:24550300).|
|Cellular Location||Late endosome. Lysosome. Early endosome Endoplasmic reticulum. Midbody. Chromosome, centromere. Note=Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network. Colocalization with early endosomes is only partial|
|Tissue Location||Highly expressed in brain, lung, kidney and liver.|
Provided below are standard protocols that you may find useful for product applications.
UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.
References for protein:
1.Liang,C., et al. Nat. Cell Biol. 8 (7), 688-699 (2006)
2.Ionov,Y., et al. Oncogene 23 (3), 639-645 (2004)
3.Goi,T., et al. Surg. Today 33 (9), 702-706 (2003)
4.Iida,A., et al. Hum. Genet. 106 (3), 277-287 (2000)
5.Perelman,B., et al. Genomics 41 (3), 397-405 (1997)
6.Bekri,S., et al. Cytogenet. Cell Genet. 79 (1-2), 125-131 (1997)
7.Teitz,T., et al. Gene 87 (2), 295-298 (1990)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
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