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UVRAG Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • IF - UVRAG Antibody (C-term) AP1850B
    Fluorescent image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1850b UVRAG (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
  • WB - UVRAG Antibody (C-term) AP1850B
    Immunoprecipitation and western blot analysis of anti-UVRAG Pab (Cat.#AP1850b) in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.
  • IHC-P - UVRAG Antibody (C-term) AP1850B
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - UVRAG Antibody (C-term) AP1850B
    Immunofluorescence staining of Autophagy UVRAG antibody (Cat# AP1850b) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P2Y5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 78151 Da
Antigen Region 666-699 aa
Additional Information
Gene ID 7405
Other Names UV radiation resistance-associated gene protein, p63, UVRAG
Target/Specificity This UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-699 amino acids from the C-terminal region of human UVRAG.
Dilution IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUVRAG Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Cellular Location Late endosome. Lysosome. Early endosome. Note=Colocalizes with RAB9-positive compartments involved in retrograde transport from late endosomes to trans-Golgi network. Colocalization with early endosomes is only partial
Tissue Location Highly expressed in brain, lung, kidney and liver.
Research Areas
Citations ( 0 )


UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.


References for protein:
1.Liang,C., et al. Nat. Cell Biol. 8 (7), 688-699 (2006)
2.Ionov,Y., et al. Oncogene 23 (3), 639-645 (2004)
3.Goi,T., et al. Surg. Today 33 (9), 702-706 (2003)
4.Iida,A., et al. Hum. Genet. 106 (3), 277-287 (2000)
5.Perelman,B., et al. Genomics 41 (3), 397-405 (1997)
6.Bekri,S., et al. Cytogenet. Cell Genet. 79 (1-2), 125-131 (1997)
7.Teitz,T., et al. Gene 87 (2), 295-298 (1990)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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$ 295.00
$ 99.00
Cat# AP1850B
(40 western blots)
Availability: In Stock
Bulk Size
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