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PI3KC3 Antibody (N-term G24)Purified Rabbit Polyclonal Antibody (Pab)
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|AP1851a||400 µl (40 western blots)||In Stock||$ 265.00||DISCONTINUED INQUIRE CLICK INQUIRE Add to cart|
|AP1851a-ev||80 µl (8 western blots)||In Stock||$ 95.00||DISCONTINED INQUIRE CLICK INQUIRE Add to cart|
- Citations : 1
PI3KC3 Antibody (N-term G24) - Product info
|Application||WB, IHC, IF|
|Other Accession||Q6AZN6, O88763, Q5D891, Q6PF93|
|Predicted||Mouse, Pig, Rat, Xenopus|
|Calculated MW||101549 Da|
PI3KC3 Antibody (N-term G24) - Additional info
|Gene ID 5289|
PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-41 amino acids from the N-terminal region of human PI3KC3.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PI3KC3 Antibody (N-term G24) is for research use only and not for use in diagnostic or therapeutic procedures.
PI3KC3 Antibody (N-term G24) - Protein Information
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity)
Ubiquitously expressed, with a highest expression in skeletal muscle
PI3KC3 Antibody (N-term G24) - Related products
PI3KC3 Antibody (N-term G24) - Application data
Western blot analysis of PI3KC3 Antibody (N-term G24)(Cat. #AP8014a) in HeLa cell lysate. PI3KC3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (N-term) (Cat.#AP1851a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term G24)(Cat#AP1851a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
PI3KC3 Antibody (N-term G24) - Research Areas
A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1.
Author : Wang J, Whiteman MW, Lian H, Wang G, Singh A, Huang D, Denmark T.
J Biol Chem. 2009 Aug 7;284(32):21412-24. doi: 10.1074/jbc.M109.026013. Epub 2009 Jun 11.
PubMed® Id : 19520853
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Provided below are standard protocols that you may find useful for product applications.
PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003).
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).