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PI3KC3 Antibody (S851)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP1851j 400 µl (40 western blots) In Stock $ 265.00 Add to cart
AP1851j-ev 80 µl (8 western blots) In Stock $ 95.00 Add to cart
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PI3KC3 Antibody (S851) - Product info

ApplicationWB, IHC
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
Primary AccessionQ8NEB9
Other AccessionQ6AZN6, O88763, Q5D891, Q6PF93
ReactivityHuman
PredictedMouse, Pig, Rat, Xenopus
IsotypeRabbit Ig
Calculated MW101549 Da

PI3KC3 Antibody (S851) - Additional info

Gene ID 5289
Other Names
PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
Target/Specificity
This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-856 amino acids from human PI3KC3.
Dilution
WB~~1:1000
IHC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
PI3KC3 Antibody (S851) is for research use only and not for use in diagnostic or therapeutic procedures.

PI3KC3 Antibody (S851) - Protein Information

Name PIK3C3
Synonyms VPS34
Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Cellular Location
Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity)
Tissue Location
Ubiquitously expressed, with a highest expression in skeletal muscle

PI3KC3 Antibody (S851) - Related products

AP1851a: PI3KC3 Antibody (N-term G24)

AP1851b: PI3KC3 Antibody (C-term E785)

AP1851e: PI3KC3 Antibody (S34)

AP1851g: PI3KC3 Antibody (S164)

AP1851h: PI3KC3 Antibody (S425)

AP3368a: Phospho-PI3KC3(S282) Antibody

AP3369a: Phospho-PI3KC3(S676) Antibody

AP3399a: Phospho-PI3KC3(S164) Antibody

AP8014a: PI3KC3 Antibody (N-term)

BP1851b: Kinase PI3KC3 Antibody (C-term) Blocking Peptide

BP1851e: PI3KC3 Antibody (S34) Blocking Peptide

BP1851g: PI3KC3 Antibody (S164) Blocking Peptide

BP1851h: PI3KC3 Antibody (S425) Blocking Peptide

BP1851j: PI3KC3 Antibody (S851) Blocking Peptide

BP3368a: Phospho-PI3KC3-S282 Antibody Blocking Peptide

BP3369a: Phospho-PI3KC3-S676 Antibody Blocking Peptide

BP3399a: Phospho-PI3KC3-S164 Antibody Blocking Peptide

BP8014a: PI3KC3 Antibody (N-term) Blocking Peptide

PI3KC3 Antibody (S851) - Application data

  • Western blot analysis of anti-PI3KC3 Antibody (S851) (Cat.#AP1851j) in CEM cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S851), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PI3KC3 Antibody (S851) - Research Areas

AntibodiesAutophagyGene OntologyBiological ProcessSignal TransductionProtein Amino Acid PhosphorylationPost-translational Protein ModificationPhosphate Metabolic ProcessPhosphorus Metabolic ProcessPhosphorylationProtein Modification ProcessBiopolymer ModificationMetabolic ProcessPrimary Metabolic ProcessProtein Metabolic ProcessCellular Protein Metabolic ProcessCellular Metabolic ProcessCellular ProcessLipid Metabolic ProcessMacromolecule Metabolic ProcessIntracellular Signaling CascadeCellular Macromolecule Metabolic ProcessCellular Lipid Metabolic ProcessGlycerolipid Metabolic ProcessOrganophosphate Metabolic ProcessLipid ModificationBiological RegulationPhospholipid Metabolic ProcessGlycerophospholipid Metabolic ProcessRegulation Of Biological ProcessSecond-messenger-mediated SignalingPhosphoinositide Metabolic ProcessRegulation Of Cellular ProcessLipid PhosphorylationPhosphoinositide PhosphorylationCellular CompartmentCytoplasmIntracellular PartIntracellularCytoplasmic PartCytosolCytosolic PartPhosphoinositide 3-kinase ComplexMacromolecular ComplexProtein ComplexMolecular FunctionCatalytic ActivityKinase ActivityPhosphotransferase Activity, Alcohol Group As AcceptorProtein Kinase ActivityTransferase Activity, Transferring Phosphorus-containing GroupsTransferase ActivityProtein BindingPurine Nucleotide BindingNucleotide BindingRibonucleotide BindingPurine Ribonucleotide BindingPurine Nucleoside BindingNucleoside BindingAdenyl Nucleotide BindingATP BindingAdenyl Ribonucleotide BindingLipid Kinase ActivityManganese Ion BindingInositol Or Phosphatidylinositol Kinase ActivityPhosphoinositide 3-kinase Activity1-phosphatidylinositol-3-kinase ActivityPathwaysKEGGInositol Phosphate MetabolismPhosphatidylinositol Signaling SystemPantherP53 PathwayHypoxia Response Via HIF ActivationAngiogenesisFGF Signaling PathwayEGF Receptor Signaling PathwayRas PathwayInsulin/IGF Pathway-protein Kinase B Signaling CascadeVEGF Signaling PathwayP53 Pathway Feedback Loops 2Endothelin Signaling PathwayPDGF Signaling PathwayTissueUterus

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

REFERENCES

Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003).
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).