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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RNF144B Antibody (Center)   

RNF144B Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RNF144B Antibody (Center) AP18585c
    RNF144B Antibody (Center) (Cat. #AP18585c) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RNF144B antibody detected the RNF144B protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7Z419
Other Accession NP_877434.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33697 Da
Antigen Region 90-118 aa
Additional Information
Gene ID 255488
Other Names E3 ubiquitin-protein ligase RNF144B, 632-, IBR domain-containing protein 2, RING finger protein 144B, p53-inducible RING finger protein, RNF144B, IBRDC2, P53RFP
Target/Specificity This RNF144B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the Central region of human RNF144B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF144B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF144B
Synonyms IBRDC2, P53RFP
Function E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
Cellular Location Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation
Tissue Location Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Research Areas
Citations (0)

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Background

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.

References

Sayan, B.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12877-12882(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Benard, G., et al. EMBO J. 29(8):1458-1471(2010)
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :

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$ 295.00
$ 99.00
Cat# AP18585c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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