RNF144B Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q7Z419 |
Other Accession | NP_877434.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 33697 Da |
Antigen Region | 90-118 aa |
Gene ID | 255488 |
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Other Names | E3 ubiquitin-protein ligase RNF144B, 632-, IBR domain-containing protein 2, RING finger protein 144B, p53-inducible RING finger protein, RNF144B, IBRDC2, P53RFP |
Target/Specificity | This RNF144B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the Central region of human RNF144B. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | RNF144B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RNF144B |
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Synonyms | IBRDC2, P53RFP |
Function | E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin- dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. |
Cellular Location | Mitochondrion membrane; Single-pass membrane protein. Cytoplasm Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation |
Tissue Location | Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis |
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Provided below are standard protocols that you may find useful for product applications.
Background
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
References
Sayan, B.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12877-12882(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Benard, G., et al. EMBO J. 29(8):1458-1471(2010)
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
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