HAS3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | O00219 |
Other Accession | O08650, NP_005320.2 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 62998 Da |
Antigen Region | 137-164 aa |
Gene ID | 3038 |
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Other Names | Hyaluronan synthase 3, Hyaluronate synthase 3, Hyaluronic acid synthase 3, HA synthase 3, HAS3 |
Target/Specificity | This HAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-164 amino acids from the Central region of human HAS3. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HAS3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HAS3 (HGNC:4820) |
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Function | Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis. |
Cellular Location | Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:O08650}; Multi-pass membrane protein. Early endosome. Note=Travels from endoplasmic reticulum (ER), Golgi to plasma membrane (PubMed:26883802). Actives only when present in plasma membrane (By similarity). O-GlcNAcylation controls its membrane localization (PubMed:26883802). A rapid recycling of HAS3 between plasma membrane and endosomes is controlled by the cytosolic levels of UDP-GlcUA and UDP-GlcNAc (PubMed:26883802) {ECO:0000250|UniProtKB:O08650, ECO:0000269|PubMed:26883802} |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. This gene is a member of the NODC/HAS gene family. Compared to the proteins encoded by other members of this gene family, this protein appears to be more of a regulator of hyaluronan synthesis. Alternative splicing results in multiple transcript variants.
References
Dunn, K.M., et al. Surgery 145(3):322-329(2009)
Nykopp, T.K., et al. BMC Cancer 9, 143 (2009) :
Nair, S., et al. J. Nephrol. 21(3):400-405(2008)
Campo, G.M., et al. Mol. Cell. Biochem. 292 (1-2), 169-178 (2006) :
Grskovic, B., et al. Biochim. Biophys. Acta 1760(6):890-895(2006)
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