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mouse CASP3 Antibody (N-term S12)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - mouse CASP3 Antibody (N-term S12) AP18708a
    Anti-CASP3 Antibody at 1:1000 dilution + mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P70677
Other Accession NP_033940.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31475 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 12367
Other Names Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, LICE, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, Casp3, Cpp32
Target/Specificity This mouse CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse CASP3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsmouse CASP3 Antibody (N-term S12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Casp3
Synonyms Cpp32
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Cellular Location Cytoplasm.
Tissue Location Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis
Research Areas
Citations (0)

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Background

CASP3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

References

Srikanth, C.V., et al. Science 330(6002):390-393(2010)
Li, F., et al. Cell Stem Cell 7(4):508-520(2010)
Wang, L., et al. J. Neurosci. 30(39):13201-13210(2010)
Gascon, E., et al. J. Neurosci. 30(37):12414-12423(2010)
Bohsali, A., et al. BMC Microbiol. 10, 237 (2010) :

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$ 295.00
$ 99.00
Cat# AP18708a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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