|Application ||WB, E|
|Other Accession||Q6ZWS8, Q7T330, Q0VCW1, Q0IHH9, Q7ZX06, NP_003554.1|
|Predicted||Xenopus, Bovine, Zebrafish, Mouse|
|Calculated MW||42132 Da|
|Antigen Region||86-115 aa|
|Other Names||Speckle-type POZ protein, HIB homolog 1, Roadkill homolog 1, SPOP|
|Target/Specificity||This SPOP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human SPOP.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPOP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.|
|Cellular Location||Nucleus. Nucleus speckle.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq].
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Zhuang, M., et al. Mol. Cell 36(1):39-50(2009)
Liu, J., et al. Science 323(5918):1218-1222(2009)
Bunce, M.W., et al. J. Biol. Chem. 283(13):8678-8686(2008)
Byun, B., et al. Biofactors 31 (3-4), 165-169 (2007) :
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