|Application ||WB, E|
|Calculated MW||114760 Da|
|Antigen Region||394-420 aa|
|Other Names||ATP-dependent RNA helicase DHX36, DEAH box protein 36, G4-resolvase 1, G4R1, MLE-like protein 1, RNA helicase associated with AU-rich element ARE, DHX36, DDX36, KIAA1488, MLEL1, RHAU|
|Target/Specificity||This DHX36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-420 amino acids from the Central region of human DHX36.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DHX36 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DDX36, KIAA1488, MLEL1, RHAU|
|Function||Proposed to have a global role in regulating mRNA expression including transcriptional regulation and mRNA stability. Binds with high affinity to and resolves tetramolecular RNA and DNA quadruplex structures. Unwinds intramolecular quadruplexes derived from the ZIC1 and the MYC promoters. Binds to quadruplex structures in the promoters of YY1 and ALPL genes and regulates their expression. Binds to telomerase RNA template component (TERC) 5'-end (nucleotides 1-43) and unwinds an internal quadruplex formation in TERC 5'-end to promote P1 helix formation; the P1 helix acts as a template boundary ensuring accurate reverse transcription and is disrupted by quadruplex formation. May be involved in regulation of telomere length. Plays a role in degradation and deadenylation of mRNAs containing in their 3'-UTR the consensus ARE sequence element. May function in sex development and spermatogenesis. May play a role in ossification.|
|Cellular Location||Nucleus. Cytoplasm. Chromosome, telomere. Note=Isoform 1 preferentially localized to the nucleus and isoform 2 localized to the cytoplasm. However, partitioning of cellular localization between the nucleus and cytoplasm is not exclusive, as isoform 1 was also detected in the cytoplasm. Both isoforms were excluded from nucleoli. Localizes to cytoplasmic stress granules|
|Tissue Location||Highly expressed in testis.|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Lattmann, S., et al. Nucleic Acids Res. 38(18):6219-6233(2010)
Kim, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15181-15186(2010)
Chalupnikova, K., et al. J. Biol. Chem. 283(50):35186-35198(2008)
Creacy, S.D., et al. J. Biol. Chem. 283(50):34626-34634(2008)
Iwamoto, F., et al. Exp. Cell Res. 314(6):1378-1391(2008)
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