|Application ||WB, E|
|Calculated MW||104753 Da|
|Antigen Region||489-517 aa|
|Other Names||G-protein coupled receptor family C group 6 member A, hGPRC6A, G-protein coupled receptor GPCR33, hGPCR33, GPRC6A|
|Target/Specificity||This GPRC6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-517 amino acids from the Central region of human GPRC6A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GPRC6A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca(2+) and Mg(2+). Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Isoform 1 is expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not detected in thymus, prostate, small intestine, tongue and colon. Isoform 1 and isoform 2 are expressed in kidney at the same level. Isoform 2 is expressed at lower level than isoform 1 in the other tissues|
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Provided below are standard protocols that you may find useful for product applications.
Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).
Giroux, S., et al. Bone 47(5):975-981(2010)
Takata, R., et al. Nat. Genet. 42(9):751-754(2010)
Pi, M., et al. J. Bone Miner. Res. 25(5):1092-1102(2010)
Dowal, L., et al. J. Biol. Chem. 281(33):23999-24014(2006)
Pi, M., et al. J. Biol. Chem. 280(48):40201-40209(2005)
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