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SCRIB Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q14160 |
| Other Accession | NP_874365.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 174915 Da |
| Antigen Region | 382-410 aa |
| Gene ID | 23513 |
|---|---|
| Other Names | Protein scribble homolog, Scribble, hScrib, Protein LAP4, SCRIB, CRIB1, KIAA0147, LAP4, SCRB1, VARTUL |
| Target/Specificity | This SCRIB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the N-terminal region of human SCRIB. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SCRIB Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SCRIB |
|---|---|
| Synonyms | CRIB1, KIAA0147, LAP4, SCRB1, VARTUL |
| Function | Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization (PubMed:15182672, PubMed:16344308, PubMed:19041750, PubMed:18716323, PubMed:18641685, PubMed:16965391, PubMed:27380321). Via its interaction with CRTAM, required for the late phase polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG and IL22 production (By similarity). Most probably functions in the establishment of apico- basal cell polarity (PubMed:16344308, PubMed:19041750). May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium (PubMed:16965391, PubMed:19041750). May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling (PubMed:18716323, PubMed:18641685). May play a role in exocytosis and in the targeting of synaptic vesicles to synapses (PubMed:15182672). Functions as an activator of Rac GTPase activity. |
| Cellular Location | Cell membrane; Peripheral membrane protein. Cell junction. Cell junction, adherens junction. Cell projection, lamellipodium. Cytoplasm. Postsynapse. Presynapse. Note=Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. In a subset of CD4+ T-cells, colocalizes with CRTAM at the immunological synapse during the late phase of T-cell activation (By similarity) {ECO:0000250|UniProtKB:Q80U72} |
| Tissue Location | Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization (summary by Nola et al., 2008 [PubMed 18716323]).
References
Liu, H., et al. J. Virol. 84(21):11164-11174(2010)
Nagasaka, K., et al. Oncogene 29(38):5311-5321(2010)
Ivanov, A.I., et al. Am. J. Pathol. 176(1):134-145(2010)
Ouyang, Z., et al. Oncol. Res. 18 (11-12), 593-599 (2010) :
Arnaud, C., et al. FEBS Lett. 583(14):2326-2332(2009)
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