|Application ||WB, E|
|Calculated MW||38287 Da|
|Antigen Region||205-234 aa|
|Other Names||DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4|
|Target/Specificity||This XRCC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-234 amino acids from the Central region of human XRCC4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||XRCC4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq].
Gomes, B.C., et al. Oncol. Rep. 24(4):1079-1085(2010)
Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Liu, N., et al. Wei Sheng Yan Jiu 39(4):407-411(2010)
Bau, D.T., et al. Anticancer Res. 30(7):2727-2730(2010)
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