|Application ||WB, E|
|Calculated MW||102487 Da|
|Antigen Region||814-843 aa|
|Other Names||Coatomer subunit beta', Beta'-coat protein, Beta'-COP, p102, COPB2|
|Target/Specificity||This COPB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 814-843 amino acids from the C-terminal region of human COPB2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||COPB2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).|
|Cellular Location||Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side Note=The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Shows only a slight preference for the cis-Golgi apparatus, compared with the trans-Golgi.|
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Provided below are standard protocols that you may find useful for product applications.
The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.
Kim, E., et al. Biochem. Biophys. Res. Commun. 395(2):244-250(2010)
Guo, Y., et al. Mol. Biol. Cell 19(7):2830-2843(2008)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
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