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DNAJB2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DNAJB2 Antibody (N-term) AP18931a
    DNAJB2 Antibody (N-term) (Cat. #AP18931a) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DNAJB2 antibody detected the DNAJB2 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25686
Other Accession Q9QYI5, NP_006727.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35580 Da
Antigen Region 42-71 aa
Additional Information
Gene ID 3300
Other Names DnaJ homolog subfamily B member 2, DnaJ protein homolog 1, Heat shock 40 kDa protein 3, Heat shock protein J1, HSJ-1, DNAJB2, HSJ1, HSPF3
Target/Specificity This DNAJB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human DNAJB2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNAJB2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNAJB2 (HGNC:5228)
Function Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family (PubMed:7957263, PubMed:22219199). In parallel, also contributes to the ubiquitin- dependent proteasomal degradation of misfolded proteins (PubMed:15936278, PubMed:21625540). Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PARK2, RHO and SOD1 and be crucial for many biological processes (PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695). Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins (PubMed:15936278).
Cellular Location Isoform 2: Cytoplasm Nucleus
Tissue Location More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level) (PubMed:1599432, PubMed:12754272). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis (at protein level) (PubMed:12754272, PubMed:1599432) Isoform 1 is more abundant in neocortex and cerebellum compared to isoform 2 (at protein level) (PubMed:12754272)
Research Areas
Citations (0)

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DNAJB2 is involved in heat shock protein and unfolded protein binding.


Claeys, K.G., et al. Am. J. Pathol. 176(6):2901-2910(2010)
Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008)
Mahoney, D.J., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 294 (6), R1901-R1910 (2008) :
Adaimy, L., et al. Am. J. Hum. Genet. 81(4):821-828(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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$ 295.00
$ 99.00
Cat# AP18931a
(40 western blots)
Availability: In Stock
Bulk Size
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