NUBP1 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P53384 |
Other Accession | Q5I050, Q3KQF0, Q6P298, Q24K00, NP_002475.2 |
Reactivity | Human |
Predicted | Bovine, Zebrafish, Xenopus |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 34534 Da |
Antigen Region | 185-212 aa |
Gene ID | 4682 |
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Other Names | Cytosolic Fe-S cluster assembly factor NUBP1 {ECO:0000255|HAMAP-Rule:MF_03038}, Nucleotide-binding protein 1 {ECO:0000255|HAMAP-Rule:MF_03038}, NBP 1 {ECO:0000255|HAMAP-Rule:MF_03038}, NUBP1 {ECO:0000255|HAMAP-Rule:MF_03038}, NBP, NBP1 |
Target/Specificity | This NUBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-212 amino acids from the Central region of human NUBP1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NUBP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NUBP1 {ECO:0000255|HAMAP-Rule:MF_03038} |
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Synonyms | NBP, NBP1 |
Function | Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery (PubMed:18573874). Required for maturation of extramitochondrial Fe-S proteins (PubMed:18573874). The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (PubMed:18573874). Implicated in the regulation of centrosome duplication (By similarity). Negatively regulates cilium formation and structure (By similarity). |
Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03038, ECO:0000269|PubMed:18573874, ECO:0000269|PubMed:29848660}. Nucleus {ECO:0000250|UniProtKB:Q9R060}. Cell projection {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000250|UniProtKB:Q9R060} Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:Q9R060}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Enriched in centrioles of microtubule asters during prophase, prometaphase and telophase stages of mitosis. Localized at centrioles and in the nucleus at interphase. Colocalizes with nubp-2 at prometaphase. Specifically localizes to the axenome of motile cilia as opposed to primary non- motile cilia. Localization is independent of NUBP2 and KIFC1 {ECO:0000250|UniProtKB:Q9R060} |
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Provided below are standard protocols that you may find useful for product applications.
Background
NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins (Nakashima et al., 1999 [PubMed 10486206]).[supplied by OMIM].
References
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009)
Hosgood, H.D. III, et al. Carcinogenesis 29(10):1938-1943(2008)
Stehling, O., et al. Mol. Cell. Biol. 28(17):5517-5528(2008)
Taniguchi, N., et al. J. Biol. Chem. 275(41):31674-31681(2000)
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