TRIM35 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9UPQ4 |
Other Accession | NP_741983.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 56540 Da |
Antigen Region | 434-463 aa |
Gene ID | 23087 |
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Other Names | Tripartite motif-containing protein 35, Hemopoietic lineage switch protein 5, TRIM35, HLS5, KIAA1098 |
Target/Specificity | This TRIM35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434-463 amino acids from the C-terminal region of human TRIM35. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TRIM35 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TRIM35 |
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Synonyms | HLS5, KIAA1098 |
Function | E3 ubiquitin-protein ligase that participates in multiple biological processes including cell death, glucose metabolism, and in particular, the innate immune response. Mediates 'Lys-63'-linked polyubiquitination of TRAF3 thereby promoting type I interferon production via RIG-I signaling pathway (PubMed:32562145). Can also catalyze 'Lys-48'-linked polyubiquitination and proteasomal degradation of viral proteins such as influenza virus PB2 (PubMed:32562145). Acts as a negative feedback regulator of TLR7- and TLR9-triggered signaling. Mechanistically, promotes the 'Lys-48'-linked ubiquitination of IRF7 and induces its degradation via a proteasome-dependent pathway (PubMed:25907537). Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth (PubMed:25263439). |
Cellular Location | Cytoplasm. Nucleus. Note=Found predominantly in cytoplasm with a granular distribution. Found in punctuate nuclear bodies (By similarity) |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified.
References
Lalonde, J.P., et al. J. Biol. Chem. 279(9):8181-8189(2004)
Kimura, F., et al. J. Biol. Chem. 278(27):25046-25054(2003)
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)
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