|Application ||WB, E|
|Calculated MW||62041 Da|
|Antigen Region||472-500 aa|
|Other Names||Cation channel sperm-associated protein 2, CatSper2, CATSPER2|
|Target/Specificity||This CATSPER2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 472-500 amino acids from the C-terminal region of human CATSPER2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CATSPER2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH.|
|Cellular Location||Cell projection, cilium, flagellum membrane; Multi-pass membrane protein Note=Specifically located in the principal piece of sperm tail|
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Provided below are standard protocols that you may find useful for product applications.
Calcium ions play a primary role in the regulation of sperm motility. This gene belongs to a family of putative cation channels that are specific to spermatozoa and localize to the flagellum. The protein family features a single repeat with six membrane-spanning segments and a predicted calcium-selective pore region. This gene is part of a tandem repeat on chromosome 15q15; the second copy of this gene is thought to be a pseudogene. Additional splice variants have been described but their full-length nature has not been determined.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Zhang, D., et al. J. Biol. Chem. 281(31):22332-22341(2006)
Clapham, D.E., et al. Pharmacol. Rev. 57(4):451-454(2005)
Quill, T.A., et al. Proc. Natl. Acad. Sci. U.S.A. 98(22):12527-12531(2001)
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