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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SPAM1 Antibody (C-term)   

SPAM1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPAM1 Antibody (C-term) AP19001b
    SPAM1 Antibody (C-term) (Cat. #AP19001b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAM1 antibody detected the SPAM1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P38567
Other Accession P38568, NP_694859.1
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57848 Da
Antigen Region 429-457 aa
Additional Information
Gene ID 6677
Other Names Hyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, SPAM1, HYAL3, PH20
Target/Specificity This SPAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-457 amino acids from the C-terminal region of human SPAM1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPAM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPAM1
Synonyms HYAL3, PH20
Function Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Testis..
Research Areas
Citations (0)

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Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].


Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Dunn, C.A., et al. BMC Genomics 6 (1), 47 (2005) :
Evans, E.A., et al. Reprod. Biol. Endocrinol. 1, 54 (2003) :
Cherr, G.N., et al. Matrix Biol. 20(8):515-525(2001)
Csoka, A.B., et al. Matrix Biol. 20(8):499-508(2001)

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Cat# AP19001b
(40 western blots)
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