|Application ||WB, E|
|Calculated MW||49642 Da|
|Antigen Region||374-400 aa|
|Other Names||Tubby-related protein 3, Tubby-like protein 3, TULP3, TUBL3|
|Target/Specificity||This TULP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-400 amino acids from the C-terminal region of human TULP3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TULP3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of the Shh signaling transduction pathway: recruited to primary cilia via association with the IFT complex A (IFT-A) and is required for recruitment of G protein- coupled receptor GPR161 to cilia, a promoter of PKA-dependent basal repression machinery in Shh signaling. Binds to phosphorylated inositide (phosphoinositide) lipids. Both IFT-A- and phosphoinositide-binding properties are required to regulate ciliary G protein-coupled receptor trafficking. Not involved in ciliogenesis.|
|Cellular Location||Nucleus. Cell membrane. Cell projection, cilium. Cytoplasm. Secreted. Note=Does not have a cleavable signal peptide and is secreted by a non- conventional pathway (By similarity). Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide- binding protein G(q) subunit alpha.|
|Tissue Location||Expressed at high levels in testis, ovaries, thyroid, and spinal chord.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the tubby gene family of bipartite transcription factors. Members of this family have been identified in plants, vertebrates, and invertebrates, and they share a conserved N-terminal transcription activation region and a conserved C-terminal DNA and phosphatidylinositol-phosphate binding region. The encoded protein binds to phosphoinositides in the plasma membrane via its C-terminal region and probably functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis, for instance, induced by G-protein-coupled-receptor signaling. It plays an important role in neuronal development and function. Two transcript variants encoding distinct isoforms have been identified for this gene.
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Ikeda, A., et al. J. Cell. Sci. 115 (PT 1), 9-14 (2002) :
Santagata, S., et al. Science 292(5524):2041-2050(2001)
Ikeda, S., et al. Invest. Ophthalmol. Vis. Sci. 40(11):2706-2712(1999)
Nishina, P.M., et al. Genomics 54(2):215-220(1998)
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