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UBE2D4 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UBE2D4 Antibody (C-term) AP19059b
    Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody (C-term)(Cat. #AP19059b). AP19059b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y2X8
Other Accession A5PKP9, NP_057067.1
Reactivity Human
Predicted Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16649 Da
Antigen Region 111-140 aa
Additional Information
Gene ID 51619
Other Names Ubiquitin-conjugating enzyme E2 D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4, UBE2D4, UBCH5D
Target/Specificity This UBE2D4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2D4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2D4
Synonyms UBCH5D
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Research Areas
Citations (0)

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Background

UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.

References

Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)

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$ 295.00
$ 99.00
Cat# AP19059b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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