- CITATIONS: 1
|Application ||WB, IF, E|
|Calculated MW||113320 Da|
|Antigen Region||294-323 aa|
|Other Names||Protein AF-10, ALL1-fused gene from chromosome 10 protein, MLLT10, AF10|
|Target/Specificity||This MLLT10 (AF10) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human MLLT10 (AF10).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MLLT10 (AF10) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA.|
|Tissue Location||Expressed abundantly in testis.|
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Translocations affecting chromosome 11q23 involve many partner chromosome regions and occur in various leukemias. The 11q23 gene involved in the translocations is MLL. MLLT10 is the partner gene to MLL1 involved in t(10;11)(p12;q23) translocations. In an analysis of two leukemia patients, the in t(10;11)(p12;q23) translocation fuses MLL1, a SET domain containg histone methyltransferase, to the MLLT10 gene. The MLLT10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain. The MLLT10 gene is one of the few MLL partner genes to be independently rearranged with a third gene in leukemia, the CALM gene in the t(10;11)(p12;q14) translocation. Chimeric fusion proteins MLL/AF10 and CALM/AF10 consistently retain the leucine zipper motif of MLLT10. The leucine zipper interacts with GAS41, a protein previously identified as the product of an amplified gene in a glioblastoma. GAS41 interacts with integrase interactor-1 (INI1), a component of the SWI/SNF complex, which acts to remodel chromatin and to modulate transcription. Retention of the leucine zipper in the MLL and CALM fusions suggested that a key feature of these chimeric proteins may be their ability to interfere in normal gene regulation through interaction with the adenosine triphosphate-dependent chromatin remodeling complexes.
Perrin, L., et al., Mol. Cell. Biol. 23(1):119-130 (2003).
Roll, P., et al., Cancer Genet. Cytogenet. 135(2):187-191 (2002).
Nakamura, T., et al., Mol. Cell 10(5):1119-1128 (2002).
Debernardi, S., et al., Blood 99(1):275-281 (2002).
Cai, Y., et al., Mol. Reprod. Dev. 61(1):126-134 (2002).
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