|Application ||WB, E|
|Calculated MW||53247 Da|
|Antigen Region||16-43 aa|
|Other Names||tRNA-splicing endonuclease subunit Sen2, tRNA-intron endonuclease Sen2, HsSen2, TSEN2, SEN2|
|Target/Specificity||This TSEN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human TSEN2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TSEN2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre- mRNA 3'-end processing factors, establishing a link between pre- tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Note=May be transiently localized in the nucleolus|
|Tissue Location||Isoform 1 and isoform 2 are widely expressed at very low level.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.
Weitzer, S., et al. Nature 447(7141):222-226(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
Paushkin, S.V., et al. Cell 117(3):311-321(2004)
Nakayama, M., et al. Genome Res. 12(11):1773-1784(2002)
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