|Application ||WB, E|
|Other Accession||O42580, Q8R1L4, O43731, Q7ZXS5, Q5U305, Q9CQM2, P33947, Q6PEH1, Q5ZKX9, Q2KJ37, Q569A6, Q99JH8, P33946, Q68ES4, Q6PAB8, NP_006792.1|
|Predicted||Xenopus, Bovine, Mouse, Rat, Chicken, Zebrafish|
|Calculated MW||24542 Da|
|Antigen Region||185-211 aa|
|Other Names||ER lumen protein-retaining receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, KDEL receptor 1, Putative MAPK-activating protein PM23, KDELR1, ERD21|
|Target/Specificity||This KDELR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the C-terminal region of human KDELR1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KDELR1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the retention of luminal endoplasmic reticulum resident proteins via vesicular recycling. This receptor recognizes the C-terminal K-D-E-L motif. COPI-coated transport intermediates, either in the form of round vesicles or as tubular processes, mediate retrograde traffic of the KDEL receptor-ligand complexes. Also required for normal vesicular traffic through the Golgi.|
|Cellular Location||Cytoplasmic vesicle, COPI-coated vesicle membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane|
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Provided below are standard protocols that you may find useful for product applications.
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Breuza, L., et al. J. Biol. Chem. 279(45):47242-47253(2004)
Bard, F., et al. J. Biol. Chem. 278(47):46601-46606(2003)
Yamamoto, K., et al. J. Biol. Chem. 278(36):34525-34532(2003)
Matsuda, A., et al. Oncogene 22(21):3307-3318(2003)
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