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MED30 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MED30 Antibody (Center) AP19149C
    MED30 Antibody (Center) (Cat. #AP19149c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MED30 antibody detected the MED30 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96HR3
Other Accession Q9CQI9, NP_542382.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20277 Da
Antigen Region 107-133 aa
Additional Information
Gene ID 90390
Other Names Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30, TRAP/Mediator complex component TRAP25, Thyroid hormone receptor-associated protein 6, Thyroid hormone receptor-associated protein complex 25 kDa component, Trap25, MED30, THRAP6, TRAP25
Target/Specificity This MED30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-133 amino acids from the Central region of human MED30.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMED30 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MED30
Synonyms THRAP6, TRAP25
Function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Cellular Location Nucleus.
Tissue Location Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle
Citations (0)

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The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).


Rienzo, M., et al. Biochim. Biophys. Acta 1799(7):487-495(2010)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)
Wang, Q., et al. J. Biol. Chem. 277(45):42852-42858(2002)

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$ 295.00
$ 99.00
Cat# AP19149C
(40 western blots)
Availability: In Stock
Bulk Size
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