|Application ||WB, E|
|Other Accession||Q9CQI9, NP_542382.1|
|Calculated MW||20277 Da|
|Antigen Region||107-133 aa|
|Other Names||Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30, TRAP/Mediator complex component TRAP25, Thyroid hormone receptor-associated protein 6, Thyroid hormone receptor-associated protein complex 25 kDa component, Trap25, MED30, THRAP6, TRAP25|
|Target/Specificity||This MED30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-133 amino acids from the Central region of human MED30.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MED30 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.|
|Tissue Location||Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).
Rienzo, M., et al. Biochim. Biophys. Acta 1799(7):487-495(2010)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)
Wang, Q., et al. J. Biol. Chem. 277(45):42852-42858(2002)
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