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MRPL28 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - MRPL28 Antibody (N-term) AP19152a
    All lanes : Anti-MRPL28 Antibody (N-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - MRPL28 Antibody (N-term) AP19152a
    Fluorescent confocal image of A431 cell stained with MRPL28 Antibody (N-term)(Cat#AP19152a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MRPL28 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MRPL28 immunoreactivity is localized to Mitochondria significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13084
Other Accession NP_006419.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30157 Da
Antigen Region 28-55 aa
Additional Information
Gene ID 10573
Other Names 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, MRPL28, MAAT1
Target/Specificity This MRPL28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-55 amino acids from the N-terminal region of human MRPL28.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMRPL28 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MRPL28
Synonyms MAAT1
Cellular Location Mitochondrion.
Tissue Location Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue
Research Areas
Citations (0)

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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.


Lamesch, P., et al. Genomics 89(3):307-315(2007)
Martin, J., et al. Nature 432(7020):988-994(2004)
Zhang, Z., et al. Genomics 81(5):468-480(2003)
Koc, E.C., et al. J. Biol. Chem. 276(47):43958-43969(2001)
Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :

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$ 295.00
$ 99.00
Cat# AP19152a
(40 western blots)
Availability: In Stock
Bulk Size
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