|Application ||WB, E|
|Other Accession||Q28029, NP_004222.2|
|Calculated MW||13370 Da|
|Antigen Region||82-111 aa|
|Other Names||V-type proton ATPase subunit F, V-ATPase subunit F, V-ATPase 14 kDa subunit, Vacuolar proton pump subunit F, ATP6V1F, ATP6S14, VATF|
|Target/Specificity||This ATP6V1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the C-terminal region of human ATP6V1F.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP6V1F Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.
Supino, R., et al. Ann. N. Y. Acad. Sci. 1171, 606-616 (2009) :
Smith, A.N., et al. J. Bioenerg. Biomembr. 40(4):371-380(2008)
Morel, N. Biol. Cell 95(7):453-457(2003)
Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)
Kawasaki-Nishi, S., et al. FEBS Lett. 545(1):76-85(2003)
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