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ATP6V1F Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP6V1F Antibody (C-term) AP19156b
    ATP6V1F Antibody (C-term) (Cat. #AP19156b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ATP6V1F antibody detected the ATP6V1F protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16864
Other Accession Q28029, NP_004222.2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13370 Da
Antigen Region 82-111 aa
Additional Information
Gene ID 9296
Other Names V-type proton ATPase subunit F, V-ATPase subunit F, V-ATPase 14 kDa subunit, Vacuolar proton pump subunit F, ATP6V1F, ATP6S14, VATF
Target/Specificity This ATP6V1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the C-terminal region of human ATP6V1F.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP6V1F Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP6V1F
Synonyms ATP6S14, VATF
Function Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Research Areas
Citations (0)

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This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.


Supino, R., et al. Ann. N. Y. Acad. Sci. 1171, 606-616 (2009) :
Smith, A.N., et al. J. Bioenerg. Biomembr. 40(4):371-380(2008)
Morel, N. Biol. Cell 95(7):453-457(2003)
Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)
Kawasaki-Nishi, S., et al. FEBS Lett. 545(1):76-85(2003)

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$ 295.00
$ 99.00
Cat# AP19156b
(40 western blots)
Availability: In Stock
Bulk Size
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