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Mouse Casc5 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, E |
|---|---|
| Primary Accession | Q66JQ7 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 179337 Da |
| Antigen Region | 1557-1583 aa |
| Other Names | Protein CASC5, Cancer susceptibility candidate gene 5 protein homolog, Kinetochore-null protein 1, Casc5, Knl1 |
|---|---|
| Target/Specificity | This Mouse Casc5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1557-1583 amino acids from the C-terminal region of mouse Casc5. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Casc5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Knl1 {ECO:0000312|MGI:MGI:1923714} |
|---|---|
| Synonyms | Casc5 |
| Function | Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (By similarity). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q8NG31}. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q8NG31} Note=Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle (By similarity) {ECO:0000250|UniProtKB:Q8NG31} |
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Background
Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (By similarity).
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