- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
RUVBL1 (TIP49) Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP1922c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
RUVBL1 (TIP49) Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | Q9Y265 |
| Other Accession | Q9DE26, P60123, P60122 |
| Reactivity | Human, Mouse |
| Predicted | Rat, Xenopus |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 50228 Da |
RUVBL1 (TIP49) Antibody (C-term) - Additional info | |
| Gene ID 8607 | |
| Other Names RUVBL1; INO80H; NMP238; TIP49; TIP49A; RuvB-like 1; 49 kDa TATA box-binding protein-interacting protein; 54 kDa erythrocyte cytosolic protein; INO80 complex subunit H; Nuclear matrix protein 238; Pontin 52; TIP49a; TIP60-associated protein 54-alpha | |
| Target/Specificity This RUVBL1 (TIP49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human RUVBL1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions RUVBL1 (TIP49) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
RUVBL1 (TIP49) Antibody (C-term) - Protein Information | |
| Name RUVBL1 | |
| Synonyms INO80H, NMP238, TIP49, TIP49A | |
| Function Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair May be able to bind plasminogen at cell surface and enhance plasminogen activation | |
| Cellular Location Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Cytoplasm, cytoskeleton, centrosome Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells | |
| Tissue Location Ubiquitously expressed with high expression in heart, skeletal muscle and testis | |
RUVBL1 (TIP49) Antibody (C-term) - Related products
AP1922a: RUVBL1 (TIP49) Antibody (Center)
AP1922c: RUVBL1 (TIP49) Antibody (C-term)
RI14671: RUVBL1 predesign siRNA
DC01206: Human RUVBL1 cDNA Clone
RUVBL1 (TIP49) Antibody (C-term) - Application data
-
Western blot analysis of RUVBL1 (arrow) using rabbit polyclonal RUVBL1 Antibody (C-term)(Cat.#AP1922c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RUVBL1 gene (Lane 2) (Origene Technologies).
RUVBL1 (TIP49) Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. High levels of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.
REFERENCES
Feng, Y., et al., Cancer Res. 63(24):8726-8734 (2003). Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999). Holzmann, K., et al., Biochem. Biophys. Res. Commun. 252(1):39-45 (1998). Bauer, A., et al., Proc. Natl. Acad. Sci. U.S.A. 95(25):14787-14792 (1998). Qiu, X.B., et al., J. Biol. Chem. 273(43):27786-27793 (1998).
