|Application ||WB, E|
|Other Accession||Q9DE27, Q9WTM5, Q2TBU9, Q6FIB9|
|Calculated MW||51157 Da|
|Antigen Region||400-430 aa|
|Other Names||RuvB-like 2, 48 kDa TATA box-binding protein-interacting protein, 48 kDa TBP-interacting protein, 51 kDa erythrocyte cytosolic protein, ECP-51, INO80 complex subunit J, Repressing pontin 52, Reptin 52, TIP49b, TIP60-associated protein 54-beta, TAP54-beta, RUVBL2, INO80J, TIP48, TIP49B|
|Target/Specificity||This Reptin/TIP49B/RUVB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-430 amino acids from the C-terminal region of human Reptin/TIP49B/RUVB2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Reptin/TIP49B/RUVB2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||INO80J, TIP48, TIP49B|
|Function||Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (5' to 3') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes.|
|Cellular Location||Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes|
|Tissue Location||Ubiquitously expressed. Highly expressed in testis and thymus|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.
Bauer, A., et al., EMBO J. 19(22):6121-6130 (2000).
Parfait, B., et al., Ann. Genet. 43(2):69-74 (2000).
Wood, M.A., et al., Mol. Cell 5(2):321-330 (2000).
Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999).
Kanemaki, M., et al., J. Biol. Chem. 274(32):22437-22444 (1999).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.